Neural Circuits, Neural Communication and Behaviour

Dale Lab, Neuroscience Research at the University of Warwick

News

Purines as an indicator of brain ischaemia:

Tian, F., Bibi, F., Dale, N. and Imray C.H.E. (2017) Blood purine measurements as a rapid real-time indicator of reversible brain ischaemia. Purinergic Signalling doi.org/10.1007/s11302-017-9578-z

Taste buds in the brain -sensing glucose and amino acids:

Lazutkaite, G., Solda, A., Lossow K., Meyerhof, W. and Dale, N. (2017) Amino acid sensing in hypothalamic tanycytes via umami taste receptors. Molecular Metabolism doi.org/10.1016/j.molmet.2017.08.015

Benford, H., Bolborea, M., Pollatzek, M., Lossow K., Hermans-Borgmeyer, I., Liu, B. Meyerhof, W., Kasparov, K. and Dale, N. (2017) A sweet taste receptor-dependent mechanism of glucosensing in hypothalamic tanycytes. Glia doi:10.1002/glia.23125.

Evolution and Cx26 hemichannels:

de Wolf L, Cook J, Dale N (2017) Evolutionary adaptation of the sensitivity of connexin26 hemichannels to CO2. Proc Roy Soc B doi:10.1098/rspb.2016.2723.

Overview

The overall theme of our group is how neurons communicate with each other to achieve the desired neural function. Our work splits into two major areas: Neurophysiology and Technology development.


Neurophysiological studies

Our interests presently concentrate around the investigation of chemical signalling in the brain. One common theme is purinergic signalling by ATP and adenosine and its roles in several different functional contexts such as: centrally mediated chemosensitive reflexes involved in the control of breathing and arousal; signalling by hypothalamic tanycytes in the context of the control of body weight and food intake; homeostatic control of sleep; endogenous neuroprotective mechanisms in the brain; and during early development. Our work goes from structural biology of proteins through to study of function in physiological systems. We use a combination of electrophysiological, imaging and biosensing methods to study these problems.


Some of our work on CO2 sensing and hypothalamic tanycytes has featured in popular articles in Physiology News.


Technology development

We have an active program to provide novel analytical tools for neuroscience research. In particular we are developing microelectrode biosensors specific for a number of transmitters to enable better exploration of chemical signalling in the nervous system. We have concentrated on microelectrode biosensors for the purines and have formed the Warwick Biosensors Group to further this aim. We have also developed biosensors for other analytes (such as glutamate, lactate, D-serine, acetylcholine) and have developed a range of highly selective biosensors for clinical diagnostic purposes. This latter work is linked to a spin out company Sarissa Biomedical Ltd specifically devoted to the commercialization of our biosensors and the development of diagnostic tools.


Banner illustration: puncta of Cx26 (red) on the surface of astrocytes (green) at the ventral surface of the medulla oblongata.